动物微生物检验技术_山东畜牧兽医职业学院

动物微生物检验技术

开课时间： 2025年03月03日 ~ 2025年06月30日

学时安排： 3-5小时每周

进行至第9周，共18周已有 1461 人参加

立即参加

课程详情

课程评价(57)

spContent=随着现代畜牧业集约化发展，动物疫病防控已成为影响现代畜牧业健康发展的制约因素，做好动物疫病防控、检疫检验，是社会公共卫生安全的重要环节。本课程通过信息化教学手段使学生具有基于课程知识，通过先进的技术、设备、手段等对常见动物疫病进行诊断、预防、控制、消毒与灭菌的核心能力。 With the intensive development of modern animal husbandry, prevention and control of animal diseases has become a restricting factor,which affect the healthy development of modern animal husbandry,prevention,quarantine and inspection is an important link of social public health security.This course enables students to have the core ability to diagnose,prevent,control,disinfect and sterilize common animal diseases through advanced technology,equipment and means based on course knowledge.

随着现代畜牧业集约化发展，动物疫病防控已成为影响现代畜牧业健康发展的制约因素，做好动物疫病防控、检疫检验，是社会公共卫生安全的重要环节。本课程通过信息化教学手段使学生具有基于课程知识，通过先进的技术、设备、手段等对常见动物疫病进行诊断、预防、控制、消毒与灭菌的核心能力。 With the intensive development of modern animal husbandry, prevention and control of animal diseases has become a restricting factor,which affect the healthy development of modern animal husbandry,prevention,quarantine and inspection is an important link of social public health security.This course enables students to have the core ability to diagnose,prevent,control,disinfect and sterilize common animal diseases through advanced technology,equipment and means based on course knowledge.

—— 课程团队

课程概述

《动物微生物检验技术》是中国特色高水平畜牧兽医专业群的专业基础课程，共64学时，4学分。落实立德树人根本任务，遵循职业教育技术技能人才成长规律，建立“项目+任务+拓展”“岗课赛证”融通课程结构模式。对接动物疫病防治等岗位群（岗），以智能化养殖场疫病防控综合性工作任务为整体，将教学内容整合为生产中的细菌病的实验室诊断（16学时）、病毒病的实验室诊断（16学时）、消毒与灭菌（8学时）、免疫防治（10学时）、免疫诊断（10学时）、生物制品及其应用（4学时）六个教学项目（课）。对接智能化养殖场岗位需求，引入动物疫病防控新方法、新技术、新规范，结合动物疫病检疫检验技能大赛等赛项标准（赛），参照兽医化验员等职业技能等级证书考核标准（证），融入思政、劳动和创新创业教育，将六个项目的教学内容重构为32个模块任务，分解岗位能力指标，细化模块化知识点151个、技能点44个。根据“一带一路”国家农牧企业人员培训等需求，增设大赛专项、课程思政、职教出海等3个拓展专项。挖掘课程思政元素，形成81个思政案例，有机融入课程内容，形成“强农兴农、求真务实”的课程思政主线。

"Animal Microbiological Examination Technology" is a basic professional course of high-level animal husbandry and veterinary professional group with Chinese characteristics, with a total of 64 hours and 4 credits. Implement the fundamental task of cultivating talents, follow the growth law of vocational education technical skills, and establish a "project + task + expansion" and "post course competition certificate" integrated course structure model. Docking post groups (posts) such as animal disease prevention and control, taking the comprehensive task of disease prevention and control in intelligent farms as a whole, The teaching content is integrated into six teaching projects (classes) : laboratory diagnosis of bacterial diseases in production (16 class hours), laboratory diagnosis of viral diseases (16 class hours), disinfection and sterilization (8 class hours), immune prevention and control (10 class hours), immune diagnosis (10 class hours), biological products and their application (4 class hours). To meet the job requirements of intelligent farms, introduce new methods, technologies and norms for animal disease prevention and control, combine the standards of animal disease quarantine and inspection skills competition (competition), refer to the assessment standards of vocational skill level certificates such as veterinary laboratory technicians, integrate ideological and political education, labor and innovation and entrepreneurship education, and restructure the teaching content of six projects into 32 module tasks. Decomposed post ability indicators, detailed 151 modular knowledge points, 44 skill points. According to the needs of personnel training of agricultural and animal husbandry enterprises in the "Belt and Road" countries, three expansion projects, including contest special, curriculum ideological and political, and vocational education going to sea, have been added. Excavating the ideological and political elements of the course, forming 81 ideological and political cases, organically integrating into the course content, forming the main line of "strengthening agriculture and rejuvenating agriculture, seeking truth and pragmatism".

授课目标

《动物微生物检验技术》课程依据专业人才培养方案拟定教学目标，要求学生学习后达到动物疫病防治员和动物检疫检验员高级工岗位需求和考核要求。

素质目标：

1.培养严谨细致的工作态度和创新求实的科学素养。

2.形成良好的生物安全防护、环境保护意识，树立人与自然和谐相处的生态文明意识。

3.提高学生的团队协作能力、创新能力和综合应用能力。

4.培养吃苦耐劳的劳动精神、自强不息的奋斗精神，传承中华优秀传统文化，增强民族自信。

5.培养胸怀天下、造福人类的家国情怀。成为“强农兴农、求真务实”高素质技术技能人才。

知识目标：

掌握细菌病、病毒病实验室诊断方法，消毒和灭菌的方法，免疫学理论知识，血清学试验、变态反应的类型及原理，兽用生物制品的种类、用途和使用注意事项。

能力目标：

1.能够利用信息化资源获取相关课程知识。

2.能根据实际病例设计动物细菌病、病毒病的实验室诊断方案，熟练操作相关实验室操作技能。

3.会对养殖环境微生物进行监测与消毒灭菌。

4.能应用免疫学理论指导传染病防治。

5.会用血清学试验或传染性变态反应进行传染病诊断、免疫监测、检疫。

6.会正确使用生物制品。

The course of Animal Microbial Inspection Technology draws up teaching objectives according to the professional personnel training plan, and requires students to meet the requirements and assessment requirements of senior positions of animal disease prevention and control personnel and animal quarantine inspectors after learning.

Quality objectives:

1. Cultivate rigorous and meticulous work attitude and innovative and realistic scientific literacy.

2. Form a good sense of biosafety protection and environmental protection, and establish an ecological civilization consciousness of harmonious coexistence between man and nature.

3. Improve students' teamwork ability, innovation ability and comprehensive application ability.

4. Cultivate the spirit of hard work and perseverance, the spirit of self-improvement, inherit the excellent traditional Chinese culture, and enhance national self-confidence.

5. Cultivate a sense of nationhood that embraces the world and benefits mankind. Become a "Strengthen agriculture, develop agriculture, seek truth and be pragmatic" high-quality technical skills personnel.

Knowledge objectives:

Master laboratory diagnostic methods of bacterial and viral diseases, methods of disinfection and sterilization, immunological theories, serological tests, types and principles of allergic reactions, types, uses and precautions of veterinary biological products.

Capability objectives:

1. Able to use information resources to acquire relevant course knowledge.

2. Able to design laboratory diagnosis schemes for animal bacterial diseases and viral diseases according to actual cases, skilled in relevant laboratory operation skills.

3. Monitoring, disinfection and sterilization of microorganisms in the breeding environment.

4. Can apply immunological theory to guide the prevention and treatment of infectious diseases.

5. Serological tests or infectious allergic reactions will be used for infectious disease diagnosis, immune surveillance and quarantine.

6. Use biological products correctly.

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课程大纲

细菌病的实验室诊断 Laboratory diagnosis of bacterial diseases

课时目标：临床细菌病诊断常用的实验室方法 Common laboratory methods for clinical bacterial disease diagnosis

1.1课程概述

1.2细菌形态和结构的认知

1.3显微镜油镜的使用及细菌形态结构的观察

1.4细菌涂片的革兰氏染色和病料触片的美蓝染色

1.5细菌生理的认知

1.6细菌的人工培养

1.7细菌的分离培养及细菌的生化试验

1.8细菌病的实验室诊断

1.1 Course Overview

1.2 Cognition of bacterial morphology and structure

1.3 The use of microscope oil lens and the observation of bacterial morphology and structure

1.4 Gram staining for bacterial smears and methylene blue staining for disease material contact sheets

1.5 Cognition of bacterial physiology

1.6 Artificial culture of bacteria

1.7 Isolation, culture and biochemical test of bacteria

1.8 Laboratory diagnosis of bacterial diseases

病毒病的实验室诊断 Laboratory diagnosis of viral diseases

课时目标：临床病毒病诊断常用的实验室方法 Common laboratory methods for the diagnosis of clinical viral diseases

2.1病毒的认知

2.2病毒形态和结构的认知

2.3病毒增殖的认知

2.4病毒的培养

2.5病毒其它特性的认知

2.6病毒的血凝试验

2.7病毒的血凝抑制试验

2.8病毒病的实验室诊断

2.1 Virus awareness

2.2 Cognition of virus morphology and structure

2.3 Cognition of virus proliferation

2.4 Culture of virus

2.5 Knowledge of other virus features

2.6 Hemagglutination test of virus

2.7 Hemagglutination inhibition test of virus

2.8 Laboratory diagnosis of viral diseases

消毒与灭菌 Disinfection and sterilization

课时目标：临床常用消毒与灭菌的方法 Clinical disinfection and sterilization methods are commonly used

3.1微生物在自然界分布的认知

3.2外界环境因素对微生物影响的认知

3.3微生物变异的认知

3.4细菌的药物敏感性试验

3.1 Cognition of the distribution of microorganisms in nature

3.2 Cognition of the influence of external environmental factors on microorganisms

3.3 Cognition of microbial variation

3.4 Drug sensitivity test of bacteria

免疫防治 Immunological control

课时目标：机体免疫系统的组成及免疫防治理论 The composition of the body immune system and the theory of immune prevention and treatment

4.1传染与免疫的认知

4.2免疫系统的认知

4.3抗原的认知

4.4免疫应答的认知

4.5体液免疫应答的认知

4.6细胞免疫应答的认知

4.7非特异性免疫应答的认知

4.1 Cognition of infection and immunity

4.2 Cognition of immune system

4.3 Antigen cognition

4.4 Cognition of immune respons

4.5 Cognition of humoral immune response

4.6 Cognition of cellular immune response

4.7 Cognition of non-specific immune response

免疫诊断 Immunologic diagnosis

课时目标：常见动物疫病的血清学诊断、变态反应诊断 Serological diagnosis and allergy diagnosis of common animal diseases

5.1血清学试验诊断

5.2鸡白痢检疫

5.3传染性法氏囊病卵黄抗体测定

5.4变态反应诊断

5.1 Serological test diagnosis

5.2 Chicken white dysentery quarantine

5.3 Determination of yolk antibody to infectious bursal disease

5.4 Allergy diagnosis

生物制品及其应用 Biological products and their applications

课时目标：疫苗、免疫血清及诊断液的应用 Application of vaccine, immune serum and diagnostic fluid

6.1生物制品的认知

6.2生物制品的应用

6.1 Cognition of biological products

6.2 Application of biological products

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预备知识

完成《动物解剖生理》《动物生物化学》等课程学习，了解基本解剖、生理、生物化学知识。

Complete courses such as Animal Anatomy and Physiology and Animal Biochemistry to understand basic anatomy, physiology and biochemistry.

参考资料

一、教材

1.授课教材

李舫，沈美艳.《动物微生物与免疫技术》.ISBN：978-7-109-26099-3.北京：中国农业出版社（第三版），2023.03（“十四五”职业教育国家规划教材），2019年8月在农业教育数字电子平台-智农书苑上线。

2.参考教材

（1）陆承平.《兽医微生物学》.北京：农业出版社，第三版，2001.（普通高等教育“十五”国家级规划教材）.

（2）邓子新，陈峰.《微生物学》.北京：高等教育出版社，第一版，2017.

（3）沈萍，陈向东.《微生物学》.北京：高等教育出版社，第八版，2016，《普通高等教育“十二五”国家规划教材》.

3.数字化教材

将传统的《动物微生物与免疫技术》纸质内容加入视频、动画、图片、PPT等资源的二维码，学生用手机扫描即可观看、学习。

一、Teaching Materials

1. Teaching materials

LI Fang,Shen Mei Yan.Animal Microbiology and Immunology.ISBN: 978-7-109-26099-3. Beijing: China Agriculture Press (3rd edition), 2023.03(the "14th Five-Year Plan" National Planning Textbook for Vocational Education), launched in August 2019 on the agricultural education digital electronic platform - Zhinong Library.

2. Refer to the textbook

(1) Lu Chengping. Veterinary Microbiology. Beijing: Agriculture Press, 3rd edition, 2001. (National Planning Textbook for the Tenth Five-Year Plan for General Higher Education).

(2) Deng Zixin, Chen Feng. Microbiology. Beijing: Higher Education Press, 1st edition, 2017.

(3) Shen Ping, Chen Xiangdong. Microbiology. Beijing: Higher Education Press, 8th Ed., 2016, "Twelfth Five-Year National Planning Textbook for General Higher Education".

3. Digital teaching materials

The traditional paper content of Animal Microbiology and Immune Technology is added to the two-dimensional code of videos, animations, pictures, PPT and other resources, and students can watch and learn by scanning it with their mobile phones.

二、信息化教学资源网站

1.国家级精品资源共享课《动物微生物》，爱课程网，网址：https://www.icourses.cn/sCourse/course_6142.html

2.畜牧兽医国家级专业教学资源库核心课程《动物微生物与免疫技术》，智慧职教，网址：https://www.icve.com.cn/portal/courseinfo?courseid=bbj5ak

3.动物检疫检验技术国家级专业教学资源库核心课程《动物微生物检验技术》，微知库，网址：https://xmzyk.36ve.com/?q=node/68139

二、Information teaching resources website

1.National high-quality goods resource sharing "animal microorganism, love course website, website: https://www.icourses.cn/sCourse/course_6142.html

2. Animal husbandry and veterinary state-level professional teaching repository core course animalmicrobes and the immune technology, vocational education wisdom, website:

https://www.icve.com.cn/portal/courseinfo?courseid=bbj5ak

3. Core course of National professional teaching resource bank of Animal Quarantine and Inspection Technology, "Animal Microbiological Inspection Technology",website:

https://xmzyk.36ve.com/?q=node/68139

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